Granzyme B (Granzyme 2, Cytotoxic T-Lymphocyte-Associated serine Esterase 1) (Gzmb) antibody

Antigen: Granzyme B (Granzyme 2, Cytotoxic T-Lymphocyte-Associated serine Esterase 1) (Gzmb)

Synonyms: HLP, CCPI, CGL1, CSPB, SECT, CGL-1, CSP-B, CTLA1, CTSGL1, GZB, CCP1, Ctla1, Ctla-1, AI553453, CCP-1/C11, RNKP-1, MGC156565, GZMB

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN100078

Additional Information

Alternative name: Granzyme B

Immunogen: A synthetic peptide from the N-terminus of human Granzyme B.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 32 kD protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned. Human granzymes B is involved in target cell apoptosis during lymphocyte-mediated cytotoxicity. This antibody is useful in the localization of granzyme B containing lytic granules and for the characterization of activated CTLs or NK cells. This antibody crossreacts with mouse and rat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against Granzyme B containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Enzymes, Metabolism

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with Granzyme B antibody