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GFP antibody (Green Fluorescent Protein) (AA 246)

Details for Product anti-GFP Antibody No. ABIN100085, Supplier: Log in to see
Antigen
Epitope
AA 246
43
27
17
13
5
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Aequorea victoria
596
5
3
2
2
2
2
1
Host
Goat
305
165
77
40
11
8
2
Clonality
Polyclonal
Conjugate
This GFP antibody is un-conjugated
31
31
26
14
12
8
8
6
5
5
5
5
4
4
4
4
4
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
502
317
159
139
122
93
63
50
32
26
13
12
9
9
8
5
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Immunogen The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Sterility Sterile filtered
Alternative Name GFP
Background Goat Anti-GFP is ideal for western blotting, ELISA, Immunohistochemistry and IP. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
UniProt P42212
Research Area Tags/Labels
Application Notes Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP-containing proteins on western blots. Researchers should determine optimal titers for applications.
Comment

Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Immunofluorescence (IF) image for anti-GFP antibody (Green Fluorescent Protein) (AA 246) (ABIN100085) antibodies-online's polyclonal anti-GFP antibody at a 1:1,000 dilution detects tau-GF...
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (AA 246) (ABIN100085) Western Blot of Rabbit anti-GFP antibody. Lane 1: HeLa cells. Lane 2: mock transfecte...
 image for anti-GFP antibody (Green Fluorescent Protein) (AA 246) (ABIN100085) anti-Green Fluorescent Protein (GFP) (AA 246) antibody (Image 3)
Product cited in: Chotard, Skorobogata, Sylvain, Shrivastava, Rocheleau: "TBC-2 Is Required for Embryonic Yolk Protein Storage and Larval Survival during L1 Diapause in Caenorhabditis elegans." in: PLoS ONE, Vol. 5, Issue 12, pp. e15662, 2011 (PubMed).

Brett, Renault, Rafalski, Webb, Brunet: "The microRNA cluster miR-106b~25 regulates adult neural stem/progenitor cell proliferation and neuronal differentiation." in: Aging, Vol. 3, Issue 2, pp. 108-24, 2011 (PubMed). Method employed by authors: Immunohistochemistry(IHC) (1:500, Sample species: Mouse (Murine)).

Xu, Leinwand, Dell, Fried-Cassorla, Raper: "The calmodulin-stimulated adenylate cyclase ADCY8 sets the sensitivity of zebrafish retinal axons to midline repellents and is required for normal midline crossing." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 21, pp. 7423-33, 2010 (PubMed).

Taura, Miura, Iwaisako, Osterreicher, Kodama, Penz-Osterreicher, Brenner: "Hepatocytes do not undergo epithelial-mesenchymal transition in liver fibrosis in mice." in: Hepatology (Baltimore, Md.), Vol. 51, Issue 3, pp. 1027-36, 2010 (PubMed).

La: "A dual promoter lentiviral vector for the in vivo evaluation of gene therapeutic approaches to axon regeneration after spinal cord injury." in: Gene therapy, Vol. 17, Issue 5, pp. 577-91, 2010 (PubMed).

Maher-Laporte, Berthiaume, Moreau, Julien, Lapointe, Mourez, DesGroseillers: "Molecular composition of staufen2-containing ribonucleoproteins in embryonic rat brain." in: PLoS ONE, Vol. 5, Issue 6, pp. e11350, 2010 (PubMed).

Hilgen, von Maltzahn, Willecke, Weiler, Dedek: "Subcellular distribution of connexin45 in OFF bipolar cells of the mouse retina." in: The Journal of comparative neurology, Vol. 519, Issue 3, pp. 433-50, 2010 (PubMed).

Puthussery, Gayet-Primo, Taylor: "Localization of the calcium-binding protein secretagogin in cone bipolar cells of the mammalian retina." in: The Journal of comparative neurology, Vol. 518, Issue 4, pp. 513-25, 2009 (PubMed).

Justice, Yuan, Sawchenko, Vale: "Type 1 corticotropin-releasing factor receptor expression reported in BAC transgenic mice: implications for reconciling ligand-receptor mismatch in the central corticotropin-releasing factor system." in: The Journal of comparative neurology, Vol. 511, Issue 4, pp. 479-96, 2008 (PubMed).

Kofidis, de Bruin, Yamane, Tanaka, Lebl, Swijnenburg, Weissman, Robbins: "Stimulation of paracrine pathways with growth factors enhances embryonic stem cell engraftment and host-specific differentiation in the heart after ischemic myocardial injury." in: Circulation, Vol. 111, Issue 19, pp. 2486-93, 2005 (PubMed).

Balsam, Wagers, Christensen, Kofidis, Weissman, Robbins: "Haematopoietic stem cells adopt mature haematopoietic fates in ischaemic myocardium." in: Nature, Vol. 428, Issue 6983, pp. 668-73, 2004 (PubMed).

Saxena, Saffery, Wong, Kalitsis, Choo: "Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated." in: The Journal of biological chemistry, Vol. 277, Issue 30, pp. 26921-6, 2002 (PubMed).