Green Fluorescent Protein (GFP) (AA 246) antibody

Details for Product No. ABIN100085
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Antigen
Epitope
AA 246
(26), (17), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Aequorea victoria
(341), (31), (11), (4), (3), (3), (2), (1), (1)
Host
Goat
(148), (147), (55), (26), (4), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(24), (19), (14), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
(300), (190), (107), (62), (59), (47), (38), (29), (29), (16), (13), (9), (8), (4), (3), (2)
Pubmed 12 references available
Catalog no. ABIN100085
Quantity 1 mg
Price
328.90 $   Plus shipping costs $45.00 and if applicable $26.40 dry ice
Options
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Sterility Sterile filtered
Alternative Name GFP
Background Goat Anti-GFP is ideal for western blotting, ELISA, Immunohistochemistry and IP. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
UniProt P42212
Research Area Tags/Labels
Application Notes Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP-containing proteins on western blots. Researchers should determine optimal titers for applications.
Comment

Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Storage -20 °C
Supplier Images
anti-Green Fluorescent Protein (GFP) (AA 246) antibody Western Blot of Rabbit anti-GFP antibody. Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 µg per lane. Primary antibody: GFP antibody at 1 µg/ml for 1 h at room temperature. Secondary antibody: IRDye® 800 conjugated Donkey-a-Goat IgG [H&L] MX7 (605-732-125) secondary antibody at 1:2,500 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 27 kDa, 33 kDa for GFP. Other band(s): none.
anti-Green Fluorescent Protein (GFP) (AA 246) antibody (2) antibodies-online's polyclonal anti-GFP antibody at a 1:1,000 dilution detects tau-GFP in cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) in Drosophila melanogaster late stage embryonic central nervous system. AlexaFluor 488™ conjugated anti-Goat antibody was used for detection at 1:300. Panel A shows a lateral view (ventral left) and Panels B and C show ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom). In all panels, anterior is up. Personal Communication, Helmata Mistry, Washington University School of Medicine, St. Louis, MO.
anti-Green Fluorescent Protein (GFP) (AA 246) antibody (3) anti-Green Fluorescent Protein (GFP) (AA 246) antibody (Image 3)
anti-Green Fluorescent Protein (GFP) (AA 246) antibody (4) anti-Green Fluorescent Protein (GFP) (AA 246) antibody (Image 4)
Product cited in: Justice, Yuan, Sawchenko et al.: "Type 1 corticotropin-releasing factor receptor expression reported in BAC transgenic mice: implications for reconciling ligand-receptor mismatch in the central corticotropin-releasing factor system." in: The Journal of comparative neurology, Vol. 511, Issue 4, pp. 479-96, 2008 (PubMed).

Puthussery, Gayet-Primo, Taylor: "Localization of the calcium-binding protein secretagogin in cone bipolar cells of the mammalian retina." in: The Journal of comparative neurology, Vol. 518, Issue 4, pp. 513-25, 2009 (PubMed).

Hilgen, von Maltzahn, Willecke et al.: "Subcellular distribution of connexin45 in OFF bipolar cells of the mouse retina." in: The Journal of comparative neurology, Vol. 519, Issue 3, pp. 433-50, 2010 (PubMed).

Brett, Renault, Rafalski et al.: "The microRNA cluster miR-106b~25 regulates adult neural stem/progenitor cell proliferation and neuronal differentiation." in: Aging, Vol. 3, Issue 2, pp. 108-24, 2011 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (1:500, Sample species: Mouse (Murine)).

General Saxena, Saffery, Wong et al.: "Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated." in: The Journal of biological chemistry, Vol. 277, Issue 30, pp. 26921-6, 2002 (PubMed).

Balsam, Wagers, Christensen et al.: "Haematopoietic stem cells adopt mature haematopoietic fates in ischaemic myocardium." in: Nature, Vol. 428, Issue 6983, pp. 668-73, 2004 (PubMed).

Kofidis, de Bruin, Yamane et al.: "Stimulation of paracrine pathways with growth factors enhances embryonic stem cell engraftment and host-specific differentiation in the heart after ischemic myocardial injury." in: Circulation, Vol. 111, Issue 19, pp. 2486-93, 2005 (PubMed).

Taura, Miura, Iwaisako et al.: "Hepatocytes do not undergo epithelial-mesenchymal transition in liver fibrosis in mice." in: Hepatology (Baltimore, Md.), Vol. 51, Issue 3, pp. 1027-36, 2010 (PubMed).

La: "A dual promoter lentiviral vector for the in vivo evaluation of gene therapeutic approaches to axon regeneration after spinal cord injury." in: Gene therapy, Vol. 17, Issue 5, pp. 577-91, 2010 (PubMed).

Xu, Leinwand, Dell et al.: "The calmodulin-stimulated adenylate cyclase ADCY8 sets the sensitivity of zebrafish retinal axons to midline repellents and is required for normal midline crossing." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 21, pp. 7423-33, 2010 (PubMed).

Maher-Laporte, Berthiaume, Moreau et al.: "Molecular composition of staufen2-containing ribonucleoproteins in embryonic rat brain." in: PLoS ONE, Vol. 5, Issue 6, pp. e11350, 2010 (PubMed).

Chotard, Skorobogata, Sylvain et al.: "TBC-2 Is Required for Embryonic Yolk Protein Storage and Larval Survival during L1 Diapause in Caenorhabditis elegans." in: PLoS ONE, Vol. 5, Issue 12, pp. e15662, 2011 (PubMed).

Hosts (148), (147), (55), (26), (4), (2), (1)
Reactivities (341), (31), (11), (4), (3), (3), (2), (1), (1)
Applications (300), (190), (107), (62), (59), (47), (38), (29), (29), (16), (13), (9), (8), (4), (3), (2)
Conjugates (24), (19), (14), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (26), (17), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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