Prolactin-Induced Protein (PIP) antibody

Antigen: Prolactin-Induced Protein (PIP)

Clonality: Monoclonal (23A3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN100124

Additional Information

Alternative name: Gross Cystic Disease Fluid Protein-15 (GCDFP15)

Immunogen: Recombinant protein encoding the excreted domain of human GCDFP-15.

Format: Supernatant (prediluted)

Isotype: IgG2a kappa

Clone: 23A3

Specificity: This antibody is specific to 15 kD monomer protein called Gross Cystic Disease Fluid Protein-15 (GCDFP-15). Gross cystic disease of the breast is benign premenopausal disorder in which cysts are predominant. These cysts appear to be formed from excessive apocrine cystic secretions, one of which is GCDFP-15. This antibody can be used in for the staining of breast carcinoma, salivary duct carcinoma and apocrine epithelia.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM Citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic

Concentration: 1 to 4 ug/ml

Purification: Supernatant (prediluted)

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Viacava, Naccarato, Bevilacqua: "Spectrum of GCDFP-15 expression in human fetal and adult normal tissues." in: Virchows Archiv : an international journal of pathology, Vol. 432, Issue 3, pp. 255-60, 1998 (PubMed).

Hall, Clements, Birrell et al.: "Prostate-specific antigen and gross cystic disease fluid protein-15 are co-expressed in androgen receptor-positive breast tumours." in: British journal of cancer, Vol. 78, Issue 3, pp. 360-5, 1998 (PubMed).