Acinus (Inducer of Chromatin Condensation) antibody

Details for Product No. ABIN1001733
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Antigen
Reactivity
Human
(3)
Host
Rabbit
(3)
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB)
(3), (3), (3)
Pubmed 2 references available
Catalog no. ABIN1001733
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days

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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Acinus antibody was raised against a peptide (ddpvrtaqvpspprgk) corresponding to amino acids 994 to 1009 of human AcinusL, 267 to 282 of human AcinusS’, or 236 to 251 of human AcinusS, which are identical to those of mouse Acinus. The selected antigenic sequence is located near the N terminus of active peptide p17.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS’.
Application Notes Acinus antibody can be used for detection of acinus by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) An approximate 220 kDa band can be detected. Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Zamzami, Kroemer: "Condensed matter in cell death." in: Nature, Vol. 401, Issue 6749, pp. 127-8, 1999 (PubMed).

Sahara, Aoto, Eguchi et al.: "Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation." in: Nature, Vol. 401, Issue 6749, pp. 168-73, 1999 (PubMed).

Hosts (3)
Reactivities (3)
Applications (3), (3), (3)
Epitopes (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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