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Details for Product No. ABIN1001735

Activating Transcription Factor 6 (ATF6) (N-Term) antibody

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Antigen
Synonyms ATF6A, ESTM49, AA789574, Atf6alpha, 9630036G24, 9130025P16Rik, ATF-6-like, GB16435, ATF6, atf6, Atf6
Epitope
»Alternatives N-Term
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
Pubmed 4 references available
Catalog no. ABIN1001735
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 7 Business Days
Immunogen ATF6 antibody was raised against a 17 amino acid peptide from near the amino terminus of human ATF6.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Activating Transcription Factor 6
Background Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR. ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes. ATF6 has two isoforms (ATF6alpha and ATF6beta), only ATF6alpha is recognized by this antibody.
Application Notes ATF6 antibody can be used for the detection of ATF6 by Western blot at 0.5 - 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Little, Ramakrishnan, Roy et al.: "The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications." in: Critical reviews in eukaryotic gene expression, Vol. 4, Issue 1, pp. 1-18, 1995 (PubMed).

Haze, Yoshida, Yanagi et al.: "Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress." in: Molecular biology of the cell, Vol. 10, Issue 11, pp. 3787-99, 1999 (PubMed).

Yoshida, Matsui, Yamamoto et al.: "XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor." in: Cell, Vol. 107, Issue 7, pp. 881-91, 2002 (PubMed).

Liu, Kaufman: "The unfolded protein response." in: Journal of cell science, Vol. 116, Issue Pt 10, pp. 1861-2, 2003 (PubMed).

Alternatives for antigen "Activating Transcription Factor 6 (ATF6)", type "Antibodies"
Hosts (69), (15), (7)
Reactivities (88), (51), (27), (13), (12), (6), (1), (1), (1)
Applications (67), (37), (27), (25), (14), (10), (8), (7), (4), (4), (4), (3), (2)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (18), (16), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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