Activation-Induced Cytidine Deaminase (AICDA) antibody

Details for Product No. ABIN1001736
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Antigen
Synonyms AID, AICDA, aid, arp2, cda2, higm2, xlaid, ARP2, CDA2, HIGM2, Aid, Arp2, 4921510D23Rik, AA409782, D6Ertd746e
Reactivity
Human, Mouse (Murine)
(99), (60), (33), (25), (5), (4), (2)
Host
Rabbit
(73), (23), (10), (8)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
(84), (53), (19), (15), (14), (12), (8), (5), (2), (1), (1)
Pubmed 4 references available
Quantity 100 μg
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days
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Catalog No. ABIN1001736
514.01 $
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Immunogen Human AID PeptideRabbit polyclol AID antibody was raised against a peptide corresponding to 14 amino acids near the carboxy-terminus of human AID (GenBank accession no. AAM95402). AID antibody is human and mouse reactive.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Activation Induced Cytidine Deaminase
Background Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.
Application Notes AID antibody can be used for detection of AID by Western blot at 1 - 2 µg/ml. (Optimal dilution should be determined by user.) Whole cell lysate from Ramos cells can be used as positive control. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far. Anti-AID is human and mouse reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Muramatsu, Sankaranand, Anant et al.: "Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells." in: The Journal of biological chemistry, Vol. 274, Issue 26, pp. 18470-6, 1999 (PubMed).

Muramatsu, Kinoshita, Fagarasan et al.: "Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme." in: Cell, Vol. 102, Issue 5, pp. 553-63, 2000 (PubMed).

Greeve, Philipsen, Krause et al.: "Expression of activation-induced cytidine deaminase in human B-cell non-Hodgkin lymphomas." in: Blood, Vol. 101, Issue 9, pp. 3574-80, 2003 (PubMed).

Cascalho: "Advantages and disadvantages of cytidine deamination." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 11, pp. 6513-8, 2004 (PubMed).

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