Amino-terminal Enhancer of Split (AES) (N-Term) antibody

Details for Product No. ABIN1001758
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Antigen
Synonyms grg, esp1, grg5, tle5, aes-1, aes-2, AES, AES-1, AES-2, ESP1, GRG, GRG5, TLE5, AL024115, Esp1, Grg, Grg-5, Grg5, Tle5, zgc:64216, xgrg-5
Epitope
N-Term
(24), (15), (7), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(53), (26), (15), (2), (1), (1), (1)
Host
Rabbit
(49), (5), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
(52), (26), (21), (8), (5), (2), (2)
Pubmed 4 references available
Quantity 100 µg
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Catalog No. ABIN1001758
514.01 $
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Immunogen Rabbit polyclol AES antibody was raised against a 16 amino acid peptide from near the amino-terminus of human AES (Genbank accession No. NP_945320).
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Amino Terminal Enhancer of Split
Background Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
Application Notes AES antibody can be used for the detection of AES by Western blot at 2 - 4 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Miyasaka, Choudhury, Hou et al.: "Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein." in: European journal of biochemistry / FEBS, Vol. 216, Issue 1, pp. 343-52, 1993 (PubMed).

Chen, Courey: "Groucho/TLE family proteins and transcriptional repression." in: Gene, Vol. 249, Issue 1-2, pp. 1-16, 2000 (PubMed).

Jan, Matter, Pai et al.: "A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors." in: Cell, Vol. 116, Issue 5, pp. 751-62, 2004 (PubMed).

Martin, Vuori: "Regulation of Bcl-2 proteins during anoikis and amorphosis." in: Biochimica et biophysica acta, Vol. 1692, Issue 2-3, pp. 145-57, 2004 (PubMed).

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