APOBEC3G (HIV Restriction Factor) (N-Term) antibody

Details for Product No. ABIN1001795, Supplier: Log in to see
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Immunogen Rabbit polyclol APOBEC3G antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G (Genbank accession No. NP_068594). APOBEC3G antibody will also detect the APOBEC3F isoform.
Blocking Peptide Blocking peptide for this product available: ABIN1004082
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5alpha which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G, a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.
Application Notes APOBEC3G antibody can be used for detection of APOBEC3G by Western blot at 5 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Stremlau, Owens, Perron, Kiessling, Autissier, Sodroski: "The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys." in: Nature, Vol. 427, Issue 6977, pp. 848-53, 2004 (PubMed).

Bieniasz: "Intrinsic immunity: a front-line defense against viral attack." in: Nature immunology, Vol. 5, Issue 11, pp. 1109-15, 2004 (PubMed).

Jarmuz, Chester, Bayliss, Gisbourne, Dunham, Scott, Navaratnam: "An anthropoid-specific locus of orphan C to U RNA-editing enzymes on chromosome 22." in: Genomics, Vol. 79, Issue 3, pp. 285-96, 2002 (PubMed).

Sheehy, Gaddis, Choi, Malim: "Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein." in: Nature, Vol. 418, Issue 6898, pp. 646-50, 2002 (PubMed).

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