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Apoptosis Inducing Factor (AIF) (Internal Region) antibody

Details for Product No. ABIN1001809, Supplier: Log in to see
Antigen
Epitope
Internal Region
4
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
11
5
3
Host
Rabbit
13
1
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
13
8
5
2
1
1
1
Supplier
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Immunogen Rabbit polyclol AIF antibody was raised against a peptide corresponding to amino acids 517 to 531 of human AIF . This sequence is identical to those of mouse and rat AIF .
Blocking Peptide Blocking peptide for this product available: ABIN1003996
Isotype IgG
Purification Ion exchange chromatography purified.
Alternative Name Apoptosis Inducing Factor
Background Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.
Pathways
Application Notes AIF antibody can be used for detection of AIF by Western blot at 0.25 to 1 µg/ml. (Optimal dilution should be determined by user.) A 67 kDa band should be detected. Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Daugas, Susin, Zamzami et al.: "Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 14, Issue 5, pp. 729-39, 2000 (PubMed).

Zamzami, Kroemer: "Condensed matter in cell death." in: Nature, Vol. 401, Issue 6749, pp. 127-8, 1999 (PubMed).

Background publications Susin, Lorenzo, Zamzami et al.: "Molecular characterization of mitochondrial apoptosis-inducing factor." in: Nature, Vol. 397, Issue 6718, pp. 441-6, 1999 (PubMed).