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Apoptosis Protease Activating Factor 1 (N-Term) antibody

Details for Product No. ABIN1001813, Supplier: Log in to see
Human, Mouse (Murine), Rat (Rattus)
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Immunogen Rabbit polyclol Apaf-1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf-1 . The sequences of the immunogenic peptide are identical between human and mouse .
Blocking Peptide Blocking peptide for this product available: ABIN1004079
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf-1 (for apoptosis protease-activating factor 1). Apaf-1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 can also associate with caspase-4 and caspase-8. Apaf-1 is ubiquitously expressed in human tissues.
Research Area Apoptosis/Necrosis
Application Notes Apaf-1 antibody can be used for detection of Apaf-1 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user.) Whole cell lysate from HeLa cells can be used as positive control and a 115 - 130 kDa band should be detected. Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far. Apaf-1 antibody is human, mouse, and rat reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Hu, Benedict, Wu et al.: "Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4386-91, 1998 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).