You are viewing an incomplete version of our website. Please click to reload the website as full version.

Apoptosis Protease Activating Factor 1 (C-Term) antibody

Details for Product No. ABIN1001814, Supplier: Log in to see
Antigen
Epitope
C-Term
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1
1
1
Host
Rabbit
1
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Immunogen Rabbit polyclol Apaf1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human Apaf1 . The sequence of the immunogenic peptide differs from that of murine Apaf1 by one amino acid.
Blocking Peptide Blocking peptide for this product available: ABIN1003735
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.
Research Area Apoptosis/Necrosis
Pathways
Application Notes This antibody can be used for detection of Apaf1 by Western blot at 0.5 – 1 myg/ml. (Optimal dilution should be determined by user.) Human heart tissue lysate can be used as positive control and a 130 kDa band should be detected. Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far. Apaf1 antibody is human, mouse and rat reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Hu, Benedict, Wu et al.: "Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4386-91, 1998 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).