Apoptosis Repressor with CARD Domain (N-Term) antibody

Details for Product No. ABIN1001816
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Human, Mouse (Murine), Rat (Rattus)
ELISA, Western Blotting (WB)
(1), (1), (1)
Pubmed 2 references available
Quantity 100 μg
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Immunogen Rabbit polyclol ARC antibody was raised against a peptide corresponding to amino acids 2 to 18 of human origin . These sequences are identical to those of human nuclear protein Nop30 and differ from those of the rat homolog of ARC by one amino acid.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK BCL10, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD . ARC interacts with caspase-2 and –8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1, and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue .
Application Notes ARC antibody can be used for detection of ARC by Western blot at 1:500 dilution. (Optimal dilution should be determined by user.) Whole cell lysate from HeLa cells can be used as positive control and an approximately 25 kDa band can be detected. Antibody can also be used for ELISA and might be suited for other applications not tested so far. It is human, mouse, and rat reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Stoss, Schwaiger, Cooper et al.: "Alternative splicing determines the intracellular localization of the novel nuclear protein Nop30 and its interaction with the splicing factor SRp30c." in: The Journal of biological chemistry, Vol. 274, Issue 16, pp. 10951-62, 1999 (PubMed).

Koseki, Inohara, Chen et al.: "ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 9, pp. 5156-60, 1998 (PubMed).

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Catalog No. ABIN1001816
$ 452.54
Plus shipping costs $45.00
100 μg
$ 452.54

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