ATG12 antibody (Autophagy Related 12) (N-Term)

Details for Product anti-ATG12 Antibody No. ABIN1001831, Supplier: Log in to see
Antigen
  • APG12
  • APG12L
  • FBR93
  • HAPG12
  • Apg12l
  • AUTOPHAGY 12
  • AUTOPHAGY 12 B
  • CG10861
  • Dmel\\CG10861
  • atg12
  • ATG12
  • apg12
  • DDBDRAFT_0185323
  • DDBDRAFT_0191412
  • DDB_0185323
  • DDB_0191412
  • 4931423H11Rik
  • A330058M13Rik
  • Atg12l
  • AO090001000593
  • autophagy related 12
  • ubiquitin-like protein ATG12B
  • Atg12p
  • autophagy protein
  • APG12/APG12B
  • autophagy associated protein Atg12
  • Autophagy-specific gene 12
  • ATG12 autophagy related 12 homolog (S. cerevisiae)
  • autophagy associated ubiquitin-like modifier Atg12
  • similar to S. cerevisiae ATG12 (YBR217W) which nucleates preautophagosome formation as a conjugate with Atg5
  • Autophagy-related protein 12 (Autophagy-related ubiquitin-like modifier ATG12)
  • autophagy protein 12
  • autophagy-related protein 12
  • ATG12
  • Atg12
  • APG12B
  • APG12
  • ARALYDRAFT_318184
  • apg12
  • atg12
  • ANI_1_922094
  • AOR_1_1064164
Alternatives
anti-Yeast ATG12 antibody for Immunoprecipitation
Epitope
N-Term
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This ATG12 antibody is un-conjugated
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Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen ATG12 antibody was raised in rabbits against a 16 amino acid peptide from near the amino terminus of human ATG12.
Blocking Peptide Blocking peptide for this product available: ABIN1004085
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name ATG12 (ATG12 Antibody Abstract)
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.
Pathways Autophagy
Application Notes ATG12 antibody can be used for the detection of ATG10 by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Hanada, Noda, Satomi, Ichimura, Fujioka, Takao, Inagaki, Ohsumi: "The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy." in: The Journal of biological chemistry, Vol. 282, Issue 52, pp. 37298-302, 2007 (PubMed).

Background publications Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

Kamada, Funakoshi, Shintani, Nagano, Ohsumi, Ohsumi: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000 (PubMed).

Kisen, Tessitore, Costelli, Gordon, Schwarze, Baccino, Seglen: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).

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