Autophagy Related 12 (ATG12) (N-Term) antibody

Details for Product No. ABIN1001831
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Antigen
Synonyms APG12, FBR93, APG12L, HAPG12, Apg12l, Atg12l, 4931423H11Rik, A330058M13Rik
Epitope
N-Term
(18), (6), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(72), (42), (42), (12), (12), (12), (4), (2)
Host
Rabbit
(73), (8), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(74), (40), (35), (13), (12), (10), (5), (1), (1), (1)
Pubmed 4 references available
Catalog no. ABIN1001831
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Immunogen ATG12 antibody was raised in rabbits against a 16 amino acid peptide from near the amino terminus of human ATG12.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name ATG12
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.
Application Notes ATG12 antibody can be used for the detection of ATG10 by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Kisen, Tessitore, Costelli et al.: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).

Kamada, Funakoshi, Shintani et al.: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000 (PubMed).

Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

Hanada, Noda, Satomi et al.: "The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy." in: The Journal of biological chemistry, Vol. 282, Issue 52, pp. 37298-302, 2007 (PubMed).

Hosts (73), (8), (3), (1)
Reactivities (72), (42), (42), (12), (12), (12), (4), (2)
Applications (74), (40), (35), (13), (12), (10), (5), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (18), (6), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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