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ATG16 (Internal Region) antibody

Details for Product No. ABIN1001832
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Antigen
Epitope
Internal Region
(6), (4), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(15), (9), (9)
Host
Rabbit
(15)
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
(15), (15), (15)
Pubmed 4 references available
Quantity 100 µg
Shipping to United States (Change)
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Catalog No. ABIN1001832
514.01 $
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Immunogen ATG16 antibody was raised in rabbits against an 18 amino acid peptide from near the center of human ATG16.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.
Application Notes ATG16 antibody can be used for the detection of ATG16 by Western blot at 0.25 – 0.5 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Kisen, Tessitore, Costelli et al.: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).

Mizushima, Noda, Ohsumi: "Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway." in: The EMBO journal, Vol. 18, Issue 14, pp. 3888-96, 1999 (PubMed).

Kamada, Funakoshi, Shintani et al.: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000 (PubMed).

Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

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