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ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae))

Details for Product anti-ATG5 Antibody No. ABIN1001834, Supplier: Login to see New
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ATG5, CG1643, DmAtg5, Dmel\\CG1643, atg5, apg5l, zgc:100934, ATATG5, AUTOPHAGY 5, MKP11.20, MKP11_20, APG5, APG5-LIKE, APG5L, ASP, hAPG5, 2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337,  ... show more
ATG5, CG1643, DmAtg5, Dmel\\CG1643, atg5, apg5l, zgc:100934, ATATG5, AUTOPHAGY 5, MKP11.20, MKP11_20, APG5, APG5-LIKE, APG5L, ASP, hAPG5, 2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337, Paddy show less
Human, Mouse (Murine), Rat (Rattus)
(247), (76), (54), (10), (7), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
(189), (42), (15), (12), (2)
This ATG5 antibody is un-conjugated
(11), (11), (10), (9), (9), (9), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(209), (117), (97), (27), (27), (11), (7), (6), (5), (5), (2), (1), (1), (1)
Pubmed 4 references available
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Quantity 100 μg
Shipping to United States ( )
Availability Will be delivered in 15 to 19 Business Days
Immunogen ATG5 antibody was raised in rabbits against a 16 amino acid peptide from near the amino terminus of human ATG5.
Blocking Peptide Blocking peptide for this product available: ABIN1004246
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name ATG5 (ATG5 Antibody Abstract)
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Three isoforms of ATG5 are known to exist, this ATG5 antibody will only detect the longest isoform.
Application Notes ATG5 antibody can be used for the detection of ATG5 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Hanada, Noda, Satomi et al.: "The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy." in: The Journal of biological chemistry, Vol. 282, Issue 52, pp. 37298-302, 2007 (PubMed).

Background publications Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

Kamada, Funakoshi, Shintani et al.: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000 (PubMed).

Kisen, Tessitore, Costelli et al.: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).

Catalog No. ABIN1001834
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