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ATR (Anthrax Toxin Receptor, Tumor Endothelial Marker 8, TEM8) (C-Term) antibody

Details for Product No. ABIN1001836, Supplier: Log in to see
Human, Mouse (Murine), Rat (Rattus)
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Immunogen Rabbit polyclol EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG (GenBank Accession number Q14249).
Blocking Peptide Blocking peptide for this product available: ABIN1004331
Isotype IgG
Purification Affinity chromatography purified via peptide column
Background The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndooG also cleaves DNA in vitro.
Application Notes EndoG antibody can be used for detection of EndoG by Western blot at 1 to 2 µg/ml. (Optimal dilution should be determined by user.) HepG2 and 3T3 cell lysates can be used as positive control and a 35 kDa band can be detected. Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far. It is human, mouse and rat reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Widlak, Li, Wang, Garrard: "Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I." in: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 48404-9, 2001

Parrish, Li, Klotz, Ledwich, Wang, Xue: "Mitochondrial endonuclease G is important for apoptosis in C. elegans." in: Nature, Vol. 412, Issue 6842, pp. 90-4, 2001

Background publications Li, Luo, Wang: "Endonuclease G is an apoptotic DNase when released from mitochondria." in: Nature, Vol. 412, Issue 6842, pp. 95-9, 2001