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Details for Product No. ABIN1001837

ATR (Anthrax Toxin Receptor, Tumor Endothelial Marker 8, TEM8) (Internal Region) antibody

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Antigen
Epitope
»Alternatives Internal Region
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Application
»Alternatives ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
Pubmed 2 references available
Catalog no. ABIN1001837
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 7 Business Days
Immunogen Rabbit polyclol Bmf antibody was raised against a synthetic peptide corresponding to 13 amino acids near the amino terminus of human Bmf (GenBank Accession number Q96LC9). This sequence differs from that of mouse by one amino acid.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.
Application Notes Bmf antibody can be used for detection of Bmf by Western blot at 2 µg/ml. (Optimal dilution should be determined by user.) HepG2 or 293 cell lysate can be used as a positive control and a band at approximately 25 kDa can be detected. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far. It is human and mouse reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Hunt, Evan: "Apoptosis. Till death us do part." in: Science (New York, N.Y.), Vol. 293, Issue 5536, pp. 1784-5, 2001 (PubMed).

Puthalakath, Villunger, OReilly et al.: "Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis." in: Science (New York, N.Y.), Vol. 293, Issue 5536, pp. 1829-32, 2001 (PubMed).

Alternatives for antigen "ATR (Anthrax Toxin Receptor, Tumor Endothelial Marker 8, TEM8)", type "Antibodies"
Hosts (1)
Reactivities (1), (1), (1)
Applications (1), (1), (1)
Epitopes (1)
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