SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1) antibody

Details for Product No. ABIN1001869
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Antigen
Synonyms im:5777505, wu:fa08f03, wu:fb13e12, MGC79086, sh3glb1, wu:fc55h11, wu:fj98d09, AA409932, AI314629, AU015566, Bif-1, mKIAA0491, PPP1R70, dJ612B15.2
Reactivity
Human, Mouse (Murine)
(50), (12), (8), (3), (1), (1), (1), (1), (1)
Host
Rabbit
(37), (9), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
(45), (21), (16), (12), (7), (5), (4), (3), (3), (2)
Pubmed 4 references available
Quantity 100 μg
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days
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Catalog No. ABIN1001869
514.01 $
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Immunogen BIF antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human BIF.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Bax Interacting Factor 1 (Endophilin B1)
Background Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family such as Bax appear to control the activation of these enzymes. Bax activity was found to be regulated by its association with Bax-interacting factor 1 (BIF), a member of the endophilin B family that is associated with intracellular membranes. Following this interaction, Bax undergoes a conformational change and translocates to mitochondrial membranes. The Bax/BIF interaction appears to be enhanced by apoptotic stimuli, suggesting that BIF acts as the trigger to activate Bax, and as suppression of BIF promoted HeLa cell colony formation in soft agar, it may have a role in the suppression of cancer progression. At least two isoforms of BIF are known to exist.
Application Notes BIF antibody can be used for the detection of BIF by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, 1993 (PubMed).

Lockshin, Osborne, Zakeri: "Cell death in the third millennium." in: Cell death and differentiation, Vol. 7, Issue 1, pp. 2-7, 2000 (PubMed).

Cuddeback, Yamaguchi, Komatsu et al.: "Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax." in: The Journal of biological chemistry, Vol. 276, Issue 23, pp. 20559-65, 2001 (PubMed).

Takahashi, Karbowski, Yamaguchi et al.: "Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis." in: Molecular and cellular biology, Vol. 25, Issue 21, pp. 9369-82, 2005 (PubMed).

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