Bcl-2 Binding Athanogene 1 (C-Term) antibody

Details for Product No. ABIN1001870, Supplier: Log in to see
Human, Mouse (Murine), Rat (Rattus)
ELISA, Western Blotting (WB)
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Immunogen BAG-1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human BAG-1.
Blocking Peptide Blocking peptide for this product available: ABIN1003746
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Bcl-2-associated athanogene 1 (BAG-1) was first identified as an anti-apoptotic bcl-2-binding protein. Later it was found to bind the molecular chaperones Hsp70 and Hsc70 through its carboxy-terminal sequence (termed the Bag domain), resulting in the inhibition of the refolding activity of these chaperones. It is thought that by binding and inhibiting these molecular chaperones, BAG-1is able to modulate the expression level of proteins requiring chaperones to fold correctly. One such group of proteins that are affected is glucocorticoid receptors. Other reports have suggested that the level of BAG-1 expression correlates with the aggressiveness of various cancers. Multiple isoforms of BAG-1 are known to exist.
Application Notes BAG-1 antibody can be used for the detection of BAG-1 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Krajewska, Turner, Shabaik, Krajewski, Reed: "Expression of BAG-1 protein correlates with aggressive behavior of prostate cancers." in: The Prostate, Vol. 66, Issue 8, pp. 801-10, 2006 (PubMed).

Cato, Mink: "BAG-1 family of cochaperones in the modulation of nuclear receptor action." in: The Journal of steroid biochemistry and molecular biology, Vol. 78, Issue 5, pp. 379-88, 2001 (PubMed).

Nollen, Brunsting, Song, Kampinga, Morimoto: "Bag1 functions in vivo as a negative regulator of Hsp70 chaperone activity." in: Molecular and cellular biology, Vol. 20, Issue 3, pp. 1083-8, 2000 (PubMed).

Background publications Takayama, Sato, Krajewski, Kochel, Irie, Millan, Reed: "Cloning and functional analysis of BAG-1: a novel Bcl-2-binding protein with anti-cell death activity." in: Cell, Vol. 80, Issue 2, pp. 279-84, 1995 (PubMed).

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