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Beclin 1 antibody (Beclin 1, Autophagy Related) (N-Term)

Details for Product anti-BECN1 Antibody No. ABIN1001880, Supplier: Log in to see
Antigen
  • beclin-1
  • atg6
  • vps30
  • beclin1
  • Beclin-1
  • becn1
  • zgc:73097
  • ATG6
  • VPS30
  • 4921513J16Rik
  • 5430417M23Rik
  • Atg6
Epitope
N-Term
34
31
19
14
13
13
13
11
11
9
8
8
8
6
6
6
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
357
101
86
14
13
11
10
6
5
3
2
1
1
1
Host
Rabbit
285
75
4
Clonality
Polyclonal
Conjugate
This Beclin 1 antibody is un-conjugated
20
19
19
19
19
19
4
4
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
283
152
125
58
56
51
27
17
15
11
6
3
1
1
Supplier
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Immunogen Rabbit polyclol Beclin-1 antibody was raised against a 17 amino acid peptide from near the amino terminus of human Beclin-1 (Genbank accession No. AAH10276).
Blocking Peptide Blocking peptide for this product available: ABIN1004095
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Beclin-1 (Autophagy Related Protein) (BECN1 Antibody Abstract)
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.
Application Notes Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5 - 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

Liang, Kleeman, Jiang et al.: "Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein." in: Journal of virology, Vol. 72, Issue 11, pp. 8586-96, 1998 (PubMed).

Kametaka, Okano, Ohsumi et al.: "Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast, Saccharomyces cerevisiae." in: The Journal of biological chemistry, Vol. 273, Issue 35, pp. 22284-91, 1998 (PubMed).

Kisen, Tessitore, Costelli et al.: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).