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beta Actin antibody (Actin, beta) (N-Term)

Details for Product anti-ACTB Antibody No. ABIN1001885, Supplier: Log in to see
Antigen
  • beta-actin
  • ps1tp5bp1
  • LOC563571
  • ACTB
  • A26C1A
  • A26C1B
  • act-2
  • actb
  • B-actin
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • BRWS1
  • PS1TP5BP1
  • ACT-5
  • Actx
  • E430023M04Rik
  • ACTA1
  • Beta-actin
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • POTE2alpha
  • POTEACTIN
Epitope
N-Term
135
39
16
15
10
7
7
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
483
208
180
51
49
41
39
32
29
22
18
13
11
10
9
8
7
5
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Host
Rabbit
316
199
18
1
1
Clonality
Polyclonal
Conjugate
This beta Actin antibody is un-conjugated
21
16
10
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
466
212
144
89
82
44
37
29
12
12
11
10
8
3
2
2
2
2
1
1
1
Supplier
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Immunogen Rabbit polyclol -actin antibody was raised against a 16 amino acid peptide from near the amino-terminus of human -actin (Genbank accession No. AAH02409).
Blocking Peptide Blocking peptide for this product available: ABIN1004096
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Beta-Actin (ACTB Antibody Abstract)
Background Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. beta-actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Application Notes b-actin antibody can be used for the detection of b-actin by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Gourlay, Ayscough: "The actin cytoskeleton: a key regulator of apoptosis and ageing?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 7, pp. 583-9, 2005 (PubMed).

Lambrechts, Van Troys, Ampe: "The actin cytoskeleton in normal and pathological cell motility." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 10, pp. 1890-909, 2004 (PubMed).