beta-Site APP Cleaving Enzyme (C-Term) antibody

Details for Product No. ABIN1001890
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Antigen
Epitope
C-Term
Reactivity
Human, Mouse (Murine)
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
Pubmed 4 references available
Quantity 100 µg
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days
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Catalog No. ABIN1001890
514.01 $
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Immunogen Rabbit polyclol BACE antibody was raised against a peptide corresponding to 17 amino acids at the carboxy terminus of human BACE .
Isotype IgG
Purification Ion exchange chromatography purified.
Background Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by two proteases. APP is first cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer’s disease. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.
Application Notes BACE can be used for detection of BACE by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) An approximately 70 kDa band should be detected. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Vassar, Bennett, Babu-Khan et al.: "Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE." in: Science (New York, N.Y.), Vol. 286, Issue 5440, pp. 735-41, 1999 (PubMed).

Yan, Bienkowski, Shuck et al.: "Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity." in: Nature, Vol. 402, Issue 6761, pp. 533-7, 1999 (PubMed).

Sinha, Anderson, Barbour et al.: "Purification and cloning of amyloid precursor protein beta-secretase from human brain." in: Nature, Vol. 402, Issue 6761, pp. 537-40, 1999 (PubMed).

Hussain, Powell, Howlett et al.: "Identification of a novel aspartic protease (Asp 2) as beta-secretase." in: Molecular and cellular neurosciences, Vol. 14, Issue 6, pp. 419-27, 2000 (PubMed).

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