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beta-Site APP-Cleaving Enzyme 2 (BACE2) (C-Term) antibody

Details for Product No. ABIN1001892, Supplier: Log in to see
Antigen
  • 1110059C24Rik
  • AEPLC
  • AI850424
  • ALP56
  • ARP1
  • ASP1
  • ASP21
  • BAE2
  • CDA13
  • CEAP1
  • DRAP
Epitope
C-Term
32
18
15
15
13
10
5
5
5
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
139
46
45
Host
Rabbit
147
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
9
8
4
4
4
3
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
118
72
38
25
17
11
8
5
3
2
2
Supplier
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Immunogen Rabbit polyclol BACE2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human BACE2 .
Blocking Peptide Blocking peptide for this product available: ABIN1003743
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name beta-Site APP Cleaving Enzyme 2 (BACE2 Antibody Abstract)
Background Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. alphabeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by proteases. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and co-localizes with APP. A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 1. BACE2 also cleaves APP at b-site and at a different site within alphabeta. BACE2 locates on chromosome 21q22.3, the so-called ‘Down critical region’, suggesting that BACE2 and alphabeta may also contribute to the pathogenesis of Down syndrome.
Application Notes BACE2 antibody can be used for detection of BACE2 by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Hussain, Powell, Howlett, Tew, Meek, Chapman, Gloger, Murphy, Southan, Ryan, Smith, Simmons, Walsh, Dingwall, Christie: "Identification of a novel aspartic protease (Asp 2) as beta-secretase." in: Molecular and cellular neurosciences, Vol. 14, Issue 6, pp. 419-27, 2000

Vassar, Bennett, Babu-Khan, Kahn, Mendiaz, Denis, Teplow, Ross, Amarante, Loeloff, Luo, Fisher, Fuller, Edenson, Lile, Jarosinski, Biere, Curran, Burgess, Louis, Collins, Treanor, Rogers, Citron: "Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE." in: Science (New York, N.Y.), Vol. 286, Issue 5440, pp. 735-41, 1999

Sinha, Anderson, Barbour, Basi, Caccavello, Davis, Doan, Dovey, Frigon, Hong, Jacobson-Croak, Jewett, Keim, Knops, Lieberburg, Power, Tan, Tatsuno, Tung, Schenk, Seubert, Suomensaari, Wang, Walker et al.: "Purification and cloning of amyloid precursor protein beta-secretase from human brain. ..." in: Nature, Vol. 402, Issue 6761, pp. 537-40, 1999

Yan, Bienkowski, Shuck, Miao, Tory, Pauley, Brashier, Stratman, Mathews, Buhl, Carter, Tomasselli, Parodi, Heinrikson, Gurney: "Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity." in: Nature, Vol. 402, Issue 6761, pp. 533-7, 1999