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BIM antibody (BCL2-Like 11 (Apoptosis Facilitator)) (Internal Region)

Details for Product anti-BCL2L11 Antibody No. ABIN1001901, Supplier: Log in to see
Antigen
  • 1500006F24Rik
  • BAM
  • Bim
  • bim
  • BIM
  • BIMEL
  • BimL
  • Bod
  • BOD
Alternatives
anti-Human BIM antibody for ELISA
Epitope
Internal Region
76
19
14
14
13
13
13
13
11
7
6
6
6
5
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
291
154
125
4
2
2
2
2
2
Host
Rabbit
249
29
15
13
7
Clonality
Polyclonal
Conjugate
This BIM antibody is un-conjugated
16
14
10
8
8
8
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
241
134
115
64
45
36
30
24
14
8
3
2
2
2
2
Supplier
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Immunogen Rabbit polyclol Bim antibody was raised against a peptide corresponding to amino acids near the center of human Bim (GenBank Accession No. NP_619527). The sequence is identical to that of mouse and rat.
Blocking Peptide Blocking peptide for this product available: ABIN1004061
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Bim (Bcl-2 Interacting Mediator of Cell Death) (BCL2L11 Antibody Abstract)
Background Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
Pathways PI3K-Akt Signaling, Neurotrophin Signaling Pathway
Application Notes Bim antibody can be used for detection of Bim by Western blot at 2.5 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications OConnor, Strasser, OReilly, Hausmann, Adams, Cory, Huang: "Bim: a novel member of the Bcl-2 family that promotes apoptosis." in: The EMBO journal, Vol. 17, Issue 2, pp. 384-95, 1998

Hsu, Lin, Hsueh: "BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members." in: Molecular endocrinology (Baltimore, Md.), Vol. 12, Issue 9, pp. 1432-40, 1998