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Bit1 / Peptidyl-tRNA Hydrolase2 (N-Term) antibody

Details for Product No. ABIN1001903
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Antigen
Epitope
N-Term
(1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(1), (1)
Host
Rabbit
(1)
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
(1), (1), (1)
Pubmed 3 references available
Quantity 100 µg
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days
Catalog No. ABIN1001903
514.01 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Rabbit polyclol Bit1 antibody was raised against a 15 amino acid peptide from near the amino-terminus of human Bit1 (Genbank accession No. NP_057161).
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
Application Notes Bit1 antibody can be used for the detection of Bit1 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Chen, Courey: "Groucho/TLE family proteins and transcriptional repression." in: Gene, Vol. 249, Issue 1-2, pp. 1-16, 2000 (PubMed).

Jan, Matter, Pai et al.: "A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors." in: Cell, Vol. 116, Issue 5, pp. 751-62, 2004 (PubMed).

Martin, Vuori: "Regulation of Bcl-2 proteins during anoikis and amorphosis." in: Biochimica et biophysica acta, Vol. 1692, Issue 2-3, pp. 145-57, 2004 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN1001903
514.01 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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