Bnip3L (Member of The Bcl-2 Family) (Internal Region) antibody

Details for Product No. ABIN1001910
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Antigen
Epitope
Internal Region
Reactivity
Human
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 4 references available
Quantity 100 μg
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Catalog No. ABIN1001910
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Immunogen Rabbit polyclol Bnip3L antibody was raised against a peptide (DAQHESGQSSSRGSSH) corresponding to amino acids 77 to 92 of human origin, which are identical to those of mouse Bnip3L .
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3alpha, and Nix (for Nip3-like protein X). Bnip3L/Bnip3alpha/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis. Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues. Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
Application Notes Bnip3L antibody can be used for detection of Bnip3L by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) The approximate 40 kDa top band represents monomer of Bnip3L (4). Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far. It is human and mouse reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Matsushima, Fujiwara, Takahashi et al.: "Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3." in: Genes, chromosomes & cancer, Vol. 21, Issue 3, pp. 230-5, 1998 (PubMed).

Chen, Cizeau, Vande Velde et al.: "Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 7-10, 1999 (PubMed).

Yasuda, Han, Dionne et al.: "BNIP3alpha: a human homolog of mitochondrial proapoptotic protein BNIP3." in: Cancer research, Vol. 59, Issue 3, pp. 533-7, 1999 (PubMed).

Imazu, Shimizu, Tagami et al.: "Bcl-2/E1B 19 kDa-interacting protein 3-like protein (Bnip3L) interacts with bcl-2/Bcl-xL and induces apoptosis by altering mitochondrial membrane permeability." in: Oncogene, Vol. 18, Issue 32, pp. 4523-9, 1999 (PubMed).

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