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BR Serine/threonine Kinase 1 (BRSK1) (C-Term) antibody

Details for Product No. ABIN1001941
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Antigen
Synonyms BRSK1, hSAD1, Gm1100, SAD-B, SADB, RGD1563268, Sad-b
Epitope
C-Term
(17), (14), (8), (7), (4), (3), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(78), (46), (33), (11), (11)
Host
Rabbit
(79), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(70), (49), (22), (15), (9), (2)
Pubmed 2 references available
Quantity 100 µg
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days
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Catalog No. ABIN1001941
514.01 $
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Immunogen BRSK1 antibody was raised in rabbits against a 28 amino acid peptide from near the carboxy terminus of human BRSK1.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name BRSK1
Background BRSK1 was initially identified as a mammalian homolog to the fission yeast S. pombe Cdr2, a mitosis-regulatory kinase and also shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK1 is unbiquitously expressed, with highest levels of expression in the brain and testes. Similar to its yeast homolog, BRSK1 is thought to be involved in stress-induced cell cycle arrest. Overexpression of this protein leads to the G2/M arrest in HeLa S2 cells and UV-induced G2/M arrest could be partially abrogated by reduced expression of BRSK1 through the use of siRNA, indicating its role in DNA damage checkpoint function. More recently, it has been shown that both BRSK1 and the related protein BRSK2 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. At least two isoforms of BRSK1 are known to exist.
Application Notes BRSK1 antibody can be used for detection of BRSK1 by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Lu, Niida, Nakanishi: "Human SAD1 kinase is involved in UV-induced DNA damage checkpoint function." in: The Journal of biological chemistry, Vol. 279, Issue 30, pp. 31164-70, 2004 (PubMed).

Kishi, Pan, Crump et al.: "Mammalian SAD kinases are required for neuronal polarization." in: Science (New York, N.Y.), Vol. 307, Issue 5711, pp. 929-32, 2005 (PubMed).

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