Bruton Agammaglobulinemia tyrosine Kinase (BTK) (N-Term) antibody

Details for Product No. ABIN1001944
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Antigen
Synonyms BTK, atk, bpk, xla, imd1, agmx1, psctk1, AGMX1, AT, ATK, BPK, IMD1, PSCTK1, XLA, AI528679, xid
Epitope
N-Term
(25), (19), (16), (14), (11), (7), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(163), (74), (68), (36), (36), (24), (7), (1)
Host
Rabbit
(126), (36)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
(126), (62), (46), (40), (35), (33), (17), (16), (2), (1)
Pubmed 4 references available
Quantity 100 µg
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days
Request Want additional data for this product?

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Catalog No. ABIN1001944
514.01 $
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Immunogen Rabbit polyclol BTK antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK (Genbank accession No. Q06187).
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Bruton's Tyrosine Kinase
Background Bruton’s tyrosine kinase (BTK) was initially identified as a member of the src family for protein-tyrosine kinases that was involved in X-linked agamma-globulinaemia, and has since been shown to involved in a number of signaling pathways in hemapoietic lineage. It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9. The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens. BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction.
Application Notes BTK antibody can be used for the detection of BTK by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Vetrie, Vorechovský, Sideras et al.: "The gene involved in X-linked agammaglobulinaemia is a member of the src family of protein-tyrosine kinases." in: Nature, Vol. 361, Issue 6409, pp. 226-33, 1993 (PubMed).

Kawakami, Kitaura, Hata et al.: "Functions of Bruton's tyrosine kinase in mast and B cells." in: Journal of leukocyte biology, Vol. 65, Issue 3, pp. 286-90, 1999 (PubMed).

Jefferies, Doyle, Brunner et al.: "Bruton's tyrosine kinase is a Toll/interleukin-1 receptor domain-binding protein that participates in nuclear factor kappaB activation by Toll-like receptor 4." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 26258-64, 2003 (PubMed).

Akira, Takeda: "Toll-like receptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 7, pp. 499-511, 2004 (PubMed).

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