Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (C-Term) antibody

Details for Product No. ABIN1001948
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Antigen
Synonyms BUB3L, hBUB3, Aa2-050, xbub3, AU019800, AU021329, AU043350, AW146323, C78067
Epitope
C-Term
(10), (4), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(50), (36), (26), (11), (1), (1), (1)
Host
Rabbit
(49), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
(41), (19), (12), (12), (9), (9), (6), (1)
Pubmed 4 references available
Quantity 100 µg
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Catalog No. ABIN1001948
514.01 $
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Immunogen Bub3 antibody was raised in rabbits against a 16 amino acid peptide from near the carboxy terminus of human bub3.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Bub3
Background The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.
Application Notes Bub3 antibody can be used for detection of bub3 by Western blot at 0.5 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Roberts, Farr, Hoyt: "The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8282-91, 1994 (PubMed).

Taylor, Ha, McKeon: "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase." in: The Journal of cell biology, Vol. 142, Issue 1, pp. 1-11, 1998 (PubMed).

Kalitsis, Earle, Fowler et al.: "Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis." in: Genes & development, Vol. 14, Issue 18, pp. 2277-82, 2000 (PubMed).

Warren, Brady, Johnston et al.: "Distinct chromosome segregation roles for spindle checkpoint proteins." in: Molecular biology of the cell, Vol. 13, Issue 9, pp. 3029-41, 2002 (PubMed).

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