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TBC1 Domain Family, Member 10C (TBC1D10C) (C-Term) antibody

Details for Product No. ABIN1001983, Supplier: Log in to see
Antigen
  • 1810062O14Rik
  • AI428527
  • CARABIN
  • EPI64C
  • RGD1311490
Epitope
C-Term
17
6
4
Reactivity
Human, Mouse (Murine)
33
16
5
5
3
2
1
1
Host
Rabbit
27
7
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
29
19
12
4
2
Supplier
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Immunogen Carabin antibody was raised in rabbits against a 16 amino acid peptide from near the carboxy terminus of human Carabin.
Blocking Peptide Blocking peptide for this product available: ABIN1003764
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Carabin (TBC1D10C Antibody Abstract)
Background Antigen binding by the T-cell receptor (TCR) is one of the critical first steps in the immune response, triggering a cascade of signaling pathways that ultimately lead to T-cell activation. Screening a yeast two-hybrid screen of a human T-cell cDNA library with calcineurin, a protein phosphatase involved in multiple signaling pathways including T-cell activation, resulted in the identification of Carabin, a member of the TBC1 domain family of proteins, as a calcineurin-binding protein. Unlike other members of the TBC1 domain protein family which are thought to have a role in regulating cell growth and differentiation, further experiments demonstrated that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway as well as an inhibitor of the Ras signaling pathway, suggesting that Carabin may also mediate crosstalk between calcineurin and Ras. Carabin antibody does not recognize TBC1D10A or TBC1D10B. Carabin is known to exist in multiple isoforms.
Application Notes Carabin antibody can be used for detection of Carabin by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Pan, Sun, Kardian, Whartenby, Pardoll, Liu: "Feedback inhibition of calcineurin and Ras by a dual inhibitory protein Carabin." in: Nature, Vol. 445, Issue 7126, pp. 433-6, 2007

Product cited in: Weil, Israël: "Deciphering the pathway from the TCR to NF-kappaB." in: Cell death and differentiation, Vol. 13, Issue 5, pp. 826-33, 2006

Im, Rao: "Activation and deactivation of gene expression by Ca2+/calcineurin-NFAT-mediated signaling." in: Molecules and cells, Vol. 18, Issue 1, pp. 1-9, 2004

White, Pasztor, Richardson, Zon: "The gene encoding TBC1D1 with homology to the tre-2/USP6 oncogene, BUB2, and cdc16 maps to mouse chromosome 5 and human chromosome 4." in: Cytogenetics and cell genetics, Vol. 89, Issue 3-4, pp. 272-5, 2000