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CARD10 antibody (Caspase Recruitment Domain Family, Member 10)

Details for Product anti-CARD10 Antibody No. ABIN1001985, Supplier: Log in to see
  • AI449026
  • Bimp1
  • BIMP1
  • CARD10
  • Carma3
  • CARMA3
Human, Mouse (Murine)
This CARD10 antibody is un-conjugated
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
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Immunogen Rabbit polyclol CARD10 antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human CARD10 (Genbank accession No. AAK26165).
Blocking Peptide Blocking peptide for this product available: ABIN1004277
Isotype IgG
Purification Immunoaffinity chromatography purified IgG
Alternative Name CARD10 (Caspase Recruitment Domain Containing Protein 10) (CARD10 Antibody Abstract)
Background Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10. This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NFkappaB. CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IkappaK complex, demonstrating its importance in the regulation of NFkappaB transcription factor activation.
Application Notes CARD10 antibody can be used for detection of CARD10 by Western blot at 5 - 10 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Fischer, Tedford, Wirth: "New roles for Bcl10 in B-cell development and LPS response." in: Trends in immunology, Vol. 25, Issue 3, pp. 113-6, 2004 (PubMed).

Stilo, Liguoro, Di Jeso, Formisano, Consiglio, Leonardi, Vito: "Physical and functional interaction of CARMA1 and CARMA3 with Ikappa kinase gamma-NFkappaB essential modulator." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34323-31, 2004 (PubMed).

Fanning, Anderson: "Protein modules as organizers of membrane structure." in: Current opinion in cell biology, Vol. 11, Issue 4, pp. 432-9, 1999 (PubMed).

Background publications Wang, Guo, Huang, Ke, Poyet, Manji, Merriam, Glucksmann, DiStefano, Alnemri, Bertin: "Card10 is a novel caspase recruitment domain/membrane-associated guanylate kinase family member that interacts with BCL10 and activates NF-kappa B." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21405-9, 2001 (PubMed).