CARMA1 (Lymphocyte Activating CARD Containing Protein) antibody

Details for Product No. ABIN1001989
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Antigen
Reactivity
Human, Mouse (Murine)
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 4 references available
Catalog no. ABIN1001989
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days

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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Rabbit polyclol CARMA1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARMA1 (Genbank accession No. Q9BXL7).
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background CARMA proteins belong to the membrane-associated guan-ylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the IK complex, thereby regulating activation of the NF-kappaB transcription factor. Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems.
Application Notes CARMA1 antibody can be used for detection of CARMA1 by Western blot at 0.5 to 2 µg/ml. (Optimal dilution should be determined by user.) A band at approximately 125 kDa can be detected. Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Fanning, Anderson: "Protein modules as organizers of membrane structure." in: Current opinion in cell biology, Vol. 11, Issue 4, pp. 432-9, 1999 (PubMed).

Bertin, Wang, Guo et al.: "CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B." in: The Journal of biological chemistry, Vol. 276, Issue 15, pp. 11877-82, 2001 (PubMed).

Gaide, Martinon, Micheau et al.: "Carma1, a CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappaB activation." in: FEBS letters, Vol. 496, Issue 2-3, pp. 121-7, 2001 (PubMed).

McAllister-Lucas, Inohara, Lucas et al.: "Bimp1, a MAGUK family member linking protein kinase C activation to Bcl10-mediated NF-kappaB induction." in: The Journal of biological chemistry, Vol. 276, Issue 33, pp. 30589-97, 2001 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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