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Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10) (C-Term) antibody

Details for Product No. ABIN1001994
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Antigen
Synonyms MCH4, ALPS2, FLICE2, xCaspase-10, alps2, caspace-10, caspase-10, flice2, mch4, xCaspace-10
Epitope
C-Term
(27), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(112), (16), (15), (1), (1), (1)
Host
Rabbit
(101), (8), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(89), (42), (23), (21), (19), (10), (10), (6), (4), (2), (1), (1)
Pubmed 3 references available
Quantity 100 µg
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days
Catalog No. ABIN1001994
514.01 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Rabbit polyclol caspase-10 antibody was raised against a peptide corresponding to amino acids 505 to 521 of human FLICE21.
Isotype IgG
Purification Antibody is purified via DEAE-column chromatography.
Alternative Name Caspase 10
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch4 and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is therefore involved in the CD95 and TNFR1 induced apoptosis1. Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8 and -9, which causes the proteolytic cleavage of many key proteins such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death. Caspase-10 is expressed in many tissues and cell lines.
Research Area Proteolysis / Ubiquitin, Apoptosis/Necrosis
Application Notes Caspase-10 antibody can be used for detection of caspase-10 by Western blot at 1:500 to 1:1000 dilution. (Optimal dilution should be determined by user.) HeLa whole cell lysate can be used as positive control and a 59 kDa band can be detected. Caspase-10 antibody only recognizes FLICE2 form of caspase-10. Antibody can also be used for ELISA and might be suited for other applications not tested so far. Caspase-10 antibody is human, mouse and rat reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Fernandes-Alnemri, Armstrong, Krebs et al.: "In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 15, pp. 7464-9, 1996 (PubMed).

Vincenz, Dixit: "Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling." in: The Journal of biological chemistry, Vol. 272, Issue 10, pp. 6578-83, 1997 (PubMed).

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN1001994
514.01 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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