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Caspase 12 (CASP12) (Internal Region) antibody

Details for Product No. ABIN1001998, Supplier: Log in to see
Antigen
  • Caspase-12
Epitope
Internal Region
16
15
14
13
12
11
9
7
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
110
95
69
Host
Rabbit
139
12
10
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
141
64
56
42
15
14
11
8
7
6
2
1
Supplier
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Immunogen Rabbit polyclol caspase-12 antibody was raised against a peptide (CTPSSPSESKRKVEDDE) corresponding to amino acids 100 to 116 of murine caspase-12.
Blocking Peptide Blocking peptide for this product available: ABIN1004009
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Caspase 12 (CASP12 Antibody Abstract)
Background Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including beta-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
Research Area Proteolysis / Ubiquitin, Apoptosis/Necrosis, Alzheimer's Disease
Application Notes Caspase-12 antibody can be used for detection of caspase-12 by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Nakagawa, Zhu, Morishima, Li, Xu, Yankner, Yuan: "Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta." in: Nature, Vol. 403, Issue 6765, pp. 98-103, 2000 (PubMed).

Mehmet: "Caspases find a new place to hide." in: Nature, Vol. 403, Issue 6765, pp. 29-30, 2000 (PubMed).

Van de Craen, Vandenabeele, Declercq, Van den Brande, Van Loo, Molemans, Schotte, Van Criekinge, Beyaert, Fiers: "Characterization of seven murine caspase family members." in: FEBS letters, Vol. 403, Issue 1, pp. 61-9, 1997 (PubMed).