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Details for Product No. ABIN1001999

Caspase 12 (CASP12) (Large Isoform) antibody

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Antigen
Synonyms Caspase-12
Epitope
»Alternatives Large Isoform
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 3 references available
Catalog no. ABIN1001999
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 7 Business Days
Immunogen Rabbit polyclol caspase-12 antibody was raised against a synthetic peptide corresponding to amino acids within the large cleavage product of murine caspase-12 (Genbank accession CAA73532).
Isotype IgG
Purification Affinity chromatography purified via peptide column
Alternative Name Caspase 12
Background Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including -secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
Research Area Proteolysis / Ubiquitin, Apoptosis/Necrosis, Alzheimer's Disease
Application Notes Caspase-12 (large) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 0.5 to 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Van de Craen, Vandenabeele, Declercq et al.: "Characterization of seven murine caspase family members." in: FEBS letters, Vol. 403, Issue 1, pp. 61-9, 1997 (PubMed).

Mehmet: "Caspases find a new place to hide." in: Nature, Vol. 403, Issue 6765, pp. 29-30, 2000 (PubMed).

Nakagawa, Zhu, Morishima et al.: "Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta." in: Nature, Vol. 403, Issue 6765, pp. 98-103, 2000 (PubMed).

Alternatives for antigen "Caspase 12 (CASP12)", type "Antibodies"
Hosts (112), (7), (3)
Reactivities (84), (83), (64)
Applications (99), (47), (37), (37), (20), (5), (5), (5), (4), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (14), (11), (7), (6), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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