Caspase 5 (Inflammatory Subfamily) (N-Term) antibody

Details for Product No. ABIN1002011
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Antigen
Epitope
N-Term
(1)
Reactivity
Human
(1)
Host
Rabbit
(1)
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB)
(1), (1), (1)
Pubmed 4 references available
Catalog no. ABIN1002011
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 7 Business Days

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Immunogen Rabbit polyclol caspase-5 antibody was raised against a 16 amino acid peptide from the amino-terminus of human caspase-5 (Genbank accession No. P51878).
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1beta and IL-18. Caspase-5 can interact with caspase-1, both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1beta. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma.
Application Notes Casp-5 antibody can be used for the detection of Caspase-5 by Western blot at 1 µg/ml. (Optimal dilution should be determined by user.) Depending on cell lines or tissues used, other cleavage products may be observed. Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Kuida, Lippke, Ku et al.: "Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme." in: Science (New York, N.Y.), Vol. 267, Issue 5206, pp. 2000-3, 1995 (PubMed).

Martinon, Burns, Tschopp: "The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta." in: Molecular cell, Vol. 10, Issue 2, pp. 417-26, 2002 (PubMed).

Gracie, Robertson, McInnes: "Interleukin-18." in: Journal of leukocyte biology, Vol. 73, Issue 2, pp. 213-24, 2003 (PubMed).

Martinon, Tschopp: "Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases." in: Cell, Vol. 117, Issue 5, pp. 561-74, 2004 (PubMed).

Hosts (1)
Reactivities (1)
Applications (1), (1), (1)
Epitopes (1)
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