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Details for Product No. ABIN1002023

Caspase 8 (Initiator Caspase) (Internal Region) antibody

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Antigen
Epitope
»Alternatives Internal Region
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN1002023
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 7 Business Days
Immunogen Caspase-8 antibody was raised against a 15 amino acid peptide from near the carboxy terminus human caspase-8 isoform E.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex. The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain, subsequent oligomerization promotes procaspase-8 autoactivation. FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis. This antibody will only detect isoform E of caspase-8.
Application Notes Casp-8 antibody can be used for the detection of Caspase-8 by Western blot at 0.5 µg/ml. (Optimal dilution should be determined by user.) Depending on cell lines or tissues used, either full-length or other cleavage products may be observed. Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Muzio, Chinnaiyan, Kischkel et al.: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996 (PubMed).

Wolf, Green: "Suicidal tendencies: apoptotic cell death by caspase family proteinases." in: The Journal of biological chemistry, Vol. 274, Issue 29, pp. 20049-52, 1999 (PubMed).

Martinon, Tschopp: "Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases." in: Cell, Vol. 117, Issue 5, pp. 561-74, 2004 (PubMed).

Zhivotovsky, Orrenius: "Caspase-2 function in response to DNA damage." in: Biochemical and biophysical research communications, Vol. 331, Issue 3, pp. 859-67, 2005 (PubMed).

Alternatives for antigen "Caspase 8 (Initiator Caspase)", type "Antibodies"
Hosts (1)
Reactivities (1), (1), (1)
Applications (1), (1), (1)
Epitopes (1)
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