Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) (Internal Region) antibody

Details for Product No. ABIN1002029
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Antigen
Synonyms APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56, AI115399, AW493809, CASP-9, Caspase-9, Mch6, Apaf3, Casp9_v1, Ice-Lap6, Casp-9-CTD, CASP9
Epitope
Internal Region
(25), (22), (18), (14), (13), (9), (7), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(298), (107), (93), (17), (15), (9), (7), (6), (3), (3), (1)
Host
Rabbit
(306), (30), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application
ELISA, Western Blotting (WB)
(284), (129), (69), (63), (51), (50), (39), (36), (10), (4), (3), (2), (1)
Pubmed 4 references available
Catalog no. ABIN1002029
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days

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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Rabbit polyclol caspase-9 antibody was raised against a peptide corresponding to amino acids 41 to 56 of human caspase-9.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Caspase 9
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
Research Area Cancer, Apoptosis/Necrosis
Application Notes Caspase-9 antibody can be used for detection of caspase-9 by Western blot at 1:1000 to 1:2000 dilution. (Optimal dilution should be determined by user.) Whole cell lysate from HeLa cells can be used as positive control and a 46 kDa band should be detected. Caspase-9 antibody has no cross response to other members in caspase family. Antibody can also be used for ELISA and might be suited for other applications not tested so far. Caspase-9 antibody is human, mouse, and rat reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Duan, Orth, Chinnaiyan et al.: "ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B." in: The Journal of biological chemistry, Vol. 271, Issue 28, pp. 16720-4, 1996 (PubMed).

Srinivasula, Fernandes-Alnemri, Zangrilli et al.: "The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 43, pp. 27099-106, 1996 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Pan, ORourke, Dixit: "Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex." in: The Journal of biological chemistry, Vol. 273, Issue 10, pp. 5841-5, 1998 (PubMed).

Hosts (306), (30), (1)
Reactivities (298), (107), (93), (17), (15), (9), (7), (6), (3), (3), (1)
Applications (284), (129), (69), (63), (51), (50), (39), (36), (10), (4), (3), (2), (1)
Conjugates (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (25), (22), (18), (14), (13), (9), (7), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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