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Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (Internal Region)

Details for Product anti-CASP9 Antibody No. ABIN1002030, Supplier: Log in to see
Antigen
  • CASP9
  • AI115399
  • APAF-3
  • AW493809
  • CASP-9
  • Caspase-9
  • ICE-LAP6
  • Mch6
  • Apaf3
  • Casp-9-CTD
  • Casp9_v1
  • Ice-Lap6
  • APAF3
  • MCH6
  • PPP1R56
  • caspase 9
  • caspase 9, apoptosis-related cysteine peptidase
  • caspase-9-like
  • CASP9
  • LOC100101592
  • Casp9
  • LOC100518913
Alternatives
anti-Human Caspase 9 antibody for Western Blotting
Epitope
Internal Region
38
23
21
17
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15
13
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11
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4
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3
3
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3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
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1
1
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1
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1
Reactivity
Human
427
152
145
24
15
11
7
6
6
3
2
1
Host
Rabbit
422
46
1
Clonality
Polyclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
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4
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4
4
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4
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3
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
402
159
142
85
74
72
53
30
23
15
13
9
6
2
2
2
Options
Supplier
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Supplier Product No.
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Immunogen Rabbit polyclol caspase-9 antibody was raised against a peptide corresponding to amino acids 299 to 318 of human caspase-9 (1-3).
Blocking Peptide Blocking peptide for this product available: ABIN1004062
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Caspase 9 (CASP9 Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Caspase-9 antibody can be used for detection of caspase-9 by Western blot at 1:1000 to 1:2000 dilution. (Optimal dilution should be determined by user.) Whole cell lysate from HeLa cells can be used as positive control and a 46 kDa band should be detected. Caspase-9 antibody has no cross response to other members in caspase family. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far. Caspase-9 antibody is human reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Duan, Orth, Chinnaiyan, Poirier, Froelich, He, Dixit: "ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B." in: The Journal of biological chemistry, Vol. 271, Issue 28, pp. 16720-4, 1996 (PubMed).