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CC-Chemokine Receptor 5 (HIV & Chemokine Receptor) (N-Term) antibody

Details for Product No. ABIN1002065, Supplier: Log in to see
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Immunogen Rabbit polyclol CCR5 antibody was raised against a peptide corresponding to amino acids 6 to 20 of human CCR5.
Blocking Peptide Blocking peptide for this product available: ABIN1004114
Isotype IgG
Purification Antibody is purified via DEAE-column chromatography.
Background Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The beta-chemokines RANTES, MIP-1alpha and MIP-1beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CXC5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.
Application Notes CCR5 antibody is human-specific and can be used for Western blot at 1:1000 to 1:2000 dilution. THP-1 whole cell lysate can be used as positive control and an approximate 40 kDa band can be detected. CCR5 antibody can also detect CCR5 by immunohistochemistry at 20 µg/ml. (Optimal dilution should be determined by user.). Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Dimitrov: "How do viruses enter cells? The HIV coreceptors teach us a lesson of complexity." in: Cell, Vol. 91, Issue 6, pp. 721-30, 1998

Littman: "Chemokine receptors: keys to AIDS pathogenesis?" in: Cell, Vol. 93, Issue 5, pp. 677-80, 1998

Deng, Liu, Ellmeier, Choe, Unutmaz, Burkhart, Di Marzio, Marmon, Sutton, Hill, Davis, Peiper, Schall, Littman, Landau: "Identification of a major co-receptor for primary isolates of HIV-1." in: Nature, Vol. 381, Issue 6584, pp. 661-6, 1996

Dragic, Litwin, Allaway, Martin, Huang, Nagashima, Cayanan, Maddon, Koup, Moore, Paxton: "HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5." in: Nature, Vol. 381, Issue 6584, pp. 667-73, 1996