Cell Death Inducing DFF-Like Effector A (C-Term) antibody

Details for Product No. ABIN1002135
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Antigen
Epitope
C-Term
(1)
Reactivity
Human
(1)
Host
Rabbit
(1)
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(1), (1), (1)
Pubmed 4 references available
Catalog no. ABIN1002135
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Immunogen Rabbit polyclol CIDE-A antibody was raised against a peptide corresponding to amino acids 200 to 217 of human CIDE-A .
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Apoptosis is related to many diseases and induced by a family of cell death receptors. Cell death signals are transduced by DD-, DED-, or CARD-containing molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase DFF40/CAD, which is chaperoned and inhibited by DFF45/ICAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is expressed in many tissues.
Application Notes CIDE-A antibody can be used for detection of CIDE-A by Western blot at 1:1000 to 1:2000 dilution. (Optimal dilution should be determined by user.) Human brain tissue lysate can be used as positive control and an approximately 23 kDa band can be detected. CIDE-A antibody has no cross activity to CIDE-B. Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far. CIDE- A is human reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).

Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998 (PubMed).

Inohara, Koseki, Chen et al.: "CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor." in: The EMBO journal, Vol. 17, Issue 9, pp. 2526-33, 1998 (PubMed).

Liu, Li, Widlak et al.: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998 (PubMed).

Hosts (1)
Reactivities (1)
Applications (1), (1), (1)
Epitopes (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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