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Details for Product No. ABIN1002139

Chk2 (Novel Check Point Kinase) (N-Term) antibody

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Antigen
Epitope
N-Term
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Host
Rabbit
Clonality Polyclonal
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
Pubmed 4 references available
Catalog no. ABIN1002139
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 7 Business Days
Immunogen Rabbit polyclol Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2 .
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2 , p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.
Application Notes Chk2 antibody can be used for detection of Chk2 by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) Jurkat or K562 cell lysate can be used as positive control and a 60 kDa band can be detected. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far. Chk2 antibody is human, mouse, and rat reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Tanaka, Arakawa, Yamaguchi et al.: "A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage." in: Nature, Vol. 404, Issue 6773, pp. 42-9, 2000 (PubMed).

Oda, Ohki, Murasawa et al.: "Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis." in: Science (New York, N.Y.), Vol. 288, Issue 5468, pp. 1053-8, 2000 (PubMed).

Oda, Arakawa, Tanaka et al.: "p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53." in: Cell, Vol. 102, Issue 6, pp. 849-62, 2000 (PubMed).

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