CIKS (NF-kB Activator) (C-Term) antibody

Details for Product No. ABIN1002145
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Antigen
Epitope
C-Term
(1)
Reactivity
Human
(1)
Host
Rabbit
(1)
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN1002145
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 7 Business Days

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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Rabbit polyclol CIKS antibody was raised against a synthetic peptide (REEEYVAPPRGPLPT) corresponding to amino acids 554 to 568 of human CIKS .
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappaB associates with IkappaB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. IkappaB is phosphorylated by IkappaB kinase (IKK) complex that contains IKKalpha, IKKbeta, and IKKgamma. A novel molecule that associates with and activates IKK was recently identified and designated CIKS (for connection to IKK and SAPK/JNK) and Act1 (for NF-kappaB activator 1). CIKS directly interacts with IKKgamma. CIKS/Act1 also activates activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). These results indicate that CIKS/Act1 is involved in the inflammation and stress responses. CIKS/Act1 is ubiquitously expressed in human tissues.
Application Notes CIKS antibody can be used for detection of CIKS by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) A band at approximately 63 kDa can be detected. Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Li, Commane, Nie et al.: "Act1, an NF-kappa B-activating protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 19, pp. 10489-93, 2000 (PubMed).

Leonardi, Chariot, Claudio et al.: "CIKS, a connection to Ikappa B kinase and stress-activated protein kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 19, pp. 10494-9, 2000 (PubMed).

Hosts (1)
Reactivities (1)
Applications (1), (1), (1)
Epitopes (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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