Death Effector Domain Containing 2 (DEDD2) antibody

Details for Product No. ABIN1002217
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Antigen
Synonyms MGC145883, DEDD2, DEDD, DKFZp459K109, FLAME-3, 2410050E11Rik
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(43), (22), (22), (12), (11), (11)
Host
Rabbit
(44)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(32), (14), (9), (5), (3), (3), (1)
Pubmed 3 references available
Quantity 100 μg
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days
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Catalog No. ABIN1002217
514.01 $
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Immunogen Rabbit polyclol DEDD2 antibody was raised against a peptide corresponding to 11 amino acids near the aminoterminus of human DEDD2 (GenBank accession no. NP_579874).
Isotype IgG
Purification Immunoaffinity chromatography purified IgG.
Alternative Name DNA Binding Death Effector Domain Containing Protein 2
Background Apoptotic signals are often triggered by cell surface death receptors through protein-protein interactions mediated by conserved domains such as the death effector domain. A novel death effector domain (DED)-containing protein, DEDD2, has been recently identified and its overexpression in transfected cells induces moderate apoptosis and results in substantial sensitization to apoptosis induced by Fas, TRAIL, and FADD. More recently, work has shown that DEDD2 can bind caspase-8 and -10 in addition to FLIP but not FADD. Like the related protein DEDD, DEDD2 translocates from the cytosol to the nucleus upon induction of apoptosis, and it has been suggested that DEDD2 may target caspase-8 to the nucleus and that DEDD2 thus plays a critical role in death receptor-induced apoptosis. At least two alternatively spliced transcript variants encoding distinct isoforms have been found for DEDD2.
Application Notes DEDD2 antibody can be used for detection of DEDD2 by Western blot at 0.5 to 1 myg/ml. (Optimal dilution should be determined by user.) RAW264.7 cell lysate can be used as a positive control. DEDD2 antibody is human, mouse and rat reactive. Western blot analysis of DEDD2 in RAW264.7 cell lysate with DEDD2 antibody at (A) 0.5, (B) 1 and (C) 2 myg/ml. Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Roth, Stenner-Liewen, Pawlowski et al.: "Identification and characterization of DEDD2, a death effector domain-containing protein." in: The Journal of biological chemistry, Vol. 277, Issue 9, pp. 7501-8, 2002 (PubMed).

Alcivar, Hu, Tang et al.: "DEDD and DEDD2 associate with caspase-8/10 and signal cell death." in: Oncogene, Vol. 22, Issue 2, pp. 291-7, 2003 (PubMed).

Tibbetts, Zheng, Lenardo: "The death effector domain protein family: regulators of cellular homeostasis." in: Nature immunology, Vol. 4, Issue 5, pp. 404-9, 2003 (PubMed).

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