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DNA Fragmentation Factor 45 (Inhibitor of CAD) (C-Term) antibody

Details for Product No. ABIN1002221, Supplier: Log in to see
Antigen
Epitope
C-Term
1
Reactivity
Human
1
Host
Rabbit
1
Clonality
Polyclonal
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
1
1
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Immunogen Rabbit polyclol DFF45 antibody was raised against a peptide corresponding to amino acids 313 to 331 of human DFF45 .
Blocking Peptide Blocking peptide for this product available: ABIN1003782
Isotype IgG
Purification Antibody is purified via DEAE-column chromatography.
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.
Application Notes DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blot (6) at 1:1000 to 1:2000 dilution and for immunoprecipitation. (Optimal dilution should be determined by user.) HeLa whole cell lysate can be used as positive control and a 45 kDa band can be detected in non-apoptotic cells. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far. DFF45 antibody is human reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Enari, Sakahira, Yokoyama, Okawa, Iwamatsu, Nagata: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).

Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998 (PubMed).

Liu, Li, Widlak, Zou, Luo, Garrard, Wang: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998 (PubMed).

Liu, Zou, Slaughter, Wang: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997 (PubMed).