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ECSIT (Intermediate in The TLR & Bmp Signaling Pathways) (C-Term) antibody

Details for Product No. ABIN1002236, Supplier: Log in to see
Antigen
Epitope
C-Term
Reactivity
Human, Mouse (Murine)
Host
Rabbit
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Supplier
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Immunogen Rabbit polyclol ECSIT antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human ECSIT (GenBank accession no. NP_057665).
Blocking Peptide Blocking peptide for this product available: ABIN1003790
Isotype IgG
Purification Ion exchange chromatography purified.
Background Activation of NF-kappaB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)-6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.
Application Notes ECSIT antibody can be used for detection of ECSIT by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Janssens, Beyaert: "Functional diversity and regulation of different interleukin-1 receptor-associated kinase (IRAK) family members." in: Molecular cell, Vol. 11, Issue 2, pp. 293-302, 2003 (PubMed).

Background publications Vogel, Fitzgerald, Fenton: "TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression." in: Molecular interventions, Vol. 3, Issue 8, pp. 466-77, 2004 (PubMed).

Takeda, Kaisho, Akira: "Toll-like receptors." in: Annual review of immunology, Vol. 21, pp. 335-76, 2003 (PubMed).