EVER1 (NT) (N-Term) antibody

Details for Product No. ABIN1002273
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Antigen
Epitope
N-Term
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 4 references available
Catalog no. ABIN1002273
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Immunogen EVER1 antibody was raised in rabbits against a 14 amino acid peptide from near the amino terminus of human EVER1.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Epidermodysplasia verruciformis (EV) is an autosomal recessive dermatosis characterized by abnormal susceptibility to human papillomaviruses (HPVs) and a high rate of progression to squamous cell carcinoma on sun-exposed skin. EV is caused by mutations in either of two adjacent genes, EVER1 and EVER2, located on chromosome 17q25.3. Both of these genes encode integral membrane proteins that localize to the endoplasmic reticulum and are predicted to form transmembrane channels. Both EVER1 and EVER2 are members of the transmembrane channel-like (TMC) protein family. EVER1 possesses eight trans-membrane domains and two leucine zipper motifs. EVER1 and EVER2 form a complex and interact with the zinc transporter 1 (ZnT-1), suggesting that EVER1 and EVER2 act to regulate cellular zinc balance. At least four isoforms of EVER1 are known to exist. This EVER1 antibody does not cross-react with EVER2.
Application Notes EVER1 antibody can be used for the detection of EVER1 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Majewski, Jabłońska, Orth: "Epidermodysplasia verruciformis. Immunological and nonimmunological surveillance mechanisms: role in tumor progression." in: Clinics in dermatology, Vol. 15, Issue 3, pp. 321-34, 1997 (PubMed).

Ramoz, Rueda, Bouadjar et al.: "Mutations in two adjacent novel genes are associated with epidermodysplasia verruciformis." in: Nature genetics, Vol. 32, Issue 4, pp. 579-81, 2002 (PubMed).

Keresztes, Mutai, Heller: "TMC and EVER genes belong to a larger novel family, the TMC gene family encoding transmembrane proteins." in: BMC genomics, Vol. 4, Issue 1, pp. 24, 2003 (PubMed).

Lazarczyk, Pons, Mendoza et al.: "Regulation of cellular zinc balance as a potential mechanism of EVER-mediated protection against pathogenesis by cutaneous oncogenic human papillomaviruses." in: The Journal of experimental medicine, Vol. 205, Issue 1, pp. 35-42, 2008 (PubMed).

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