FLICE Inhibitory Protein (CFLAR) (C-Term) antibody

Details for Product No. ABIN1002317
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Antigen
Epitope
C-Term
(2), (1)
Reactivity
Mouse (Murine)
(3), (2), (2)
Host
Rabbit
(3)
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
(3), (3), (3)
Pubmed 4 references available
Quantity 100 μg
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Catalog No. ABIN1002317
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Immunogen Rabbit polyclol mFLIP antibody was raised against a peptide corresponding to amino acids 449 to 465 of mouse FLIPL/CASH.
Isotype IgG
Purification Antibody is purified via DEAE-column chromatography.
Alternative Name FLICE Inhibitory Protein
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.
Application Notes mFLIP antibody can be used for the detection of FLIP by Western blot at 1:500 to 1:1000 dilution. (Optimal dilution should be determined by user.) NIH/3T3 whole cell lysate can be used as positive control and a 55 kDa band can be detected. FLIP has short form (FLIPS) and long form (FLIPL), and this mFLIP antibody recognizes the FLIPL only. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far. This antibody is mouse reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Thome, Schneider, Hofmann et al.: "Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors." in: Nature, Vol. 386, Issue 6624, pp. 517-21, 1997 (PubMed).

Shu, Halpin, Goeddel: "Casper is a FADD- and caspase-related inducer of apoptosis." in: Immunity, Vol. 6, Issue 6, pp. 751-63, 1997 (PubMed).

Hu, Vincenz, Ni et al.: "I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 28, pp. 17255-7, 1997 (PubMed).

Irmler, Thome, Hahne et al.: "Inhibition of death receptor signals by cellular FLIP." in: Nature, Vol. 388, Issue 6638, pp. 190-5, 1997 (PubMed).

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